Laboratory Automation Workstation

Laboratory Automation Workstation

PCR sawv cev rau Polymerase Chain Reaction, uas tau tsim los txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau nws PCR muaj ntau yam kev siv xws li tshuaj mus rau kev kawm ib puag ncig mus rau kev tshawb nrhiav kev ua phem. Vim tias kev kis kab mob sib kis thaum lub sijhawm ua haujlwm PCR tuaj yeem tsim cov txiaj ntsig tsis raug, yuav tsum muaj ib puag ncig huv. PCR cov chaw ua haujlwm tiv thaiv keeb kwm yav dhau los thiab hla kev sib kis los ntawm kev ua kom tag nrho cov pa paug hauv huab cua kom deb ntawm cov qauv. Lawv mus los ntawm ntau lub npe: PCR hoods, huv lub rooj zaum, laminar flow hoods, thiab cov npe mus rau.

Khoom Taw qhia
PRCXI: Koj tus kws tshaj lij PCR Kev Tsim Kho Chaw Ua Haujlwm Chaw Muag Khoom!

PRCXI Bioinformatics Co., Ltd. yog lub chaw tsim khoom ntawm pipetting workstations nyob rau hauv Suzhou, Suav. Peb lub tuam txhab tau tsim nyob rau xyoo 2014, nrog rau 17, 000-square-meter niaj hnub R&D chaw thiab pab neeg ua haujlwm zoo, tau tsim thawj lub tshuab ua haujlwm hauv tsev ua ntej ua haujlwm nrog cov qauv ywj pheej. Tam sim no, peb cov khoom tseem ceeb yog pipetting workstations, suav nrog SC9000 phau ntawv pipetting workstation, SC9100 semi-automatic pipetting workstation thiab SC9320 tag nrho tsis siv neeg pipetting workstation, as well as match magnetic stands, adapters and functional modules.

Cov khoom lag luam nplua nuj

Peb cov khoom lag luam muaj nplua nuj heev, suav nrog kev ua haujlwm siab micro-kua ua cov txheej txheem, siab tsis siv neeg lub khob ntim tshuab thiab cov tshuab tsis siv neeg nucleic acid extraction, nrog rau ntau yam kev txhawb nqa khoom siv thiab kev siv thev naus laus zis.

Khoom zoo

Peb lub Hoobkas muaj kev ua pwm, kev sim, CNC ua, cov ntawv hlau ua, kev sib dhos ua haujlwm, thiab lwm yam, thiab tau nruab nrog cov khoom siv ntau lawm xws li Taican precision machine, Huaqun tshuab cuab yeej, STAR SB20R G hom, thiab lwm yam.

 

Ntau tus khub

Peb tau tsim kev sib raug zoo nrog ntau tus neeg koom tes hauv kev lag luam, suav nrog WuXi AppTec, DIAN Diagnostics, Mgi Tech, thiab cov koom haum tshawb fawb sawv cev los ntawm Tsinghua University.

Quality Assurance

Tag nrho peb cov khoom tau raug soj ntsuam ua haujlwm thiab kev kuaj xyuas zoo tom qab tsim khoom, thiab ua raws li ISO, CE thiab lwm yam ntawv pov thawj, thiab muaj ntau daim ntawv pov thawj kev ntsuas zoo.

 

Peb cov khoom muaj feem xyuam

 

1 Channel Automatic Workstaion

Channel Automatic Workstation

Ib qho kev siv lub tshuab pipetting, tseem hu ua "kua tuav neeg hlau", yog ib qho cuab yeej uas ua kom ceev cov txheej txheem ntawm kev thauj cov kua ntim cov kua. Ib qho channel pipette yog ib lub cuab yeej kuaj uas tuaj yeem hloov tau ib qho aliquot ib zaug. Lawv feem ntau siv nyob rau hauv lub chaw soj nstuam nrog tsawg tus qauv throughput, xws li cov kev tshawb fawb thiab kev loj hlob.

Pre Electrophoresis Loading Workstation

Ua ntej Electrophoresis Loading Workstation

Pre-loading yog ib hom gel electrophoresis stain uas tshwm sim ua ntej khiav ib gel. Nws suav nrog ntxiv ib qho stain rau qhov chaw thau khoom thiab sib xyaw nrog DNA. Electrophoresis yog cov txheej txheem kuaj siv los cais DNA, RNA lossis cov protein molecules raws li lawv qhov loj me thiab cov nqi hluav taws xob.

Serial Dilution Workstation

Serial Dilution Workstation

Lub serial dilution workstation yog ib qho cuab yeej uas tso cai rau cov neeg siv los ua qhov kev ntsuas ntsuas hauv ib kab lossis kab qauv. Serial dilution yog txheej txheem siv nyob rau hauv lub chaw soj nstuam los kwv yees qhov concentration ntawm cov qauv los yog kom tau qhov xav tau concentration ntawm ib tug reagent, tshuaj, los yog compound. Nws suav nrog diluting tus qauv, reagent, tshuaj lom neeg, lossis cov khoom sib xyaw nrog qhov ua kom tsis tu ncua.

Dual Module Workstations

Dual Module Workstations

Lub chaw ua haujlwm ob lub raj xa dej yog lub chaw soj nstuam uas tso cai rau cov kws tshawb fawb los hloov cov ntim tshwj xeeb ntawm cov qauv lossis cov reagent. Nws tuaj yeem siv rau ntau daim ntawv thov nyob rau hauv lub chaw soj nstuam uas tsuas muaj me me throughput. Nws ua rau tus neeg siv tau sai thiab yooj yim sau ntau daim phiaj siv rau hauv cov ntaub so ntswg kab lis kev cai, tshuaj xyuas tshuaj, lossis tshuaj ntsuam enzyme.

Pipetting Robots

Pipetting Robots

Pipetting robots, tseem hu ua kua tuav neeg hlau, yog cov tshuab uas tuaj yeem hloov cov kua me me ntawm cov ntim. Lawv tuaj yeem pab nrog kev ua haujlwm hauv chaw kuaj mob niaj hnub, pab tiv thaiv kev sib kis los ntawm kev siv tshuab ntxhua khaub ncaws.

Liquid Handling Automated Workstation

Liquid Handling Automated Workstation

Lub tshuab ua kua siv lub tshuab ua haujlwm yog lub tshuab uas siv lub tshuab ua haujlwm los sib xyaw, faib, thiab ua kua dej. Nws ua kom cov txheej txheem pipetting thiab dispensing thaum tib lub sij hawm nce qhov tseeb ntawm kev ua haujlwm rau ntau hom kua thiab ntim. Los ntawm kev siv lub tshuab ua kua, koj tau txais kev ntxhov siab heev ntawm kev tuav tuav.

Automated Liquid Handler

Automated Liquid Handler

Cov cuab yeej siv ua kua siv tau tuaj yeem tuaj yeem ua tau ntau yam sib txawv thiab ntau thiab tsawg los siv rau hauv ntau qhov chaw. Cov tshuab no siv lub cev muaj zog los yog lub koob txhaj tshuaj, txuas nrog lub caj npab robotic, kom faib cov ntim cov kua los yog cov qauv mus rau lub thawv ntim.

Laboratory Automation Workstation

Laboratory Automation Workstation

Tsis siv neeg pipetting systems poob nyob rau hauv qeb ntawm cov cuab yeej ua kua, uas feem ntau yog siv rau kev luam, aliquoting, pooling thiab mixing thiab serial dilution ntawm cov kua. Cov txheej txheem automated pipetting yog siv los ua kom cov txheej txheem thauj me me thiab meej ntim cov kua.

Nucleic And Extraction Workstation

Nucleic Thiab Extraction Workstation

Lub chaw haujlwm nucleic acid extraction workstation yog cov cuab yeej kho mob uas tuaj yeem ua kev kuaj mob hauv vitro. Nws siv cov cuab yeej siv tshuab, hluav taws xob, thiab software thev naus laus zis los ua kom muaj kev rho tawm ntawm nucleic acids. Automated systems siv hlau nplaum-pob zeb raws li cov cuab yeej siv los ua kom cov lysing, ntxuav, thiab elution cov kauj ruam ntawm kev rho tawm.

 

PCR System Construction Workstation yog dab tsi?

 

 

PCR sawv cev rau Polymerase Chain Reaction, uas tau tsim los txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau nws PCR muaj ntau yam kev siv xws li tshuaj mus rau kev kawm ib puag ncig mus rau kev tshawb nrhiav kev ua phem. Vim tias kev kis kab mob sib kis thaum lub sijhawm ua haujlwm PCR tuaj yeem tsim cov txiaj ntsig tsis raug, yuav tsum muaj ib puag ncig huv. PCR cov chaw ua haujlwm tiv thaiv keeb kwm yav dhau los thiab hla kev sib kis los ntawm kev ua kom tag nrho cov pa paug hauv huab cua kom deb ntawm cov qauv. Lawv mus los ntawm ntau lub npe: PCR hoods, huv lub rooj zaum, laminar flow hoods, thiab cov npe mus rau.

 

Cov yam ntxwv ntawm PCR System Construction Workstation
 

Kev tu kom zoo
Peb cov PCR chaw ua haujlwm muaj cov HEPA lim uas ua kom ceev ceev dual huv los ntawm kev ua kom tsis muaj pa hauv huab cua thiab ntawm qhov chaw los tiv thaiv kev loj hlob, kab mob thiab pwm, muab qhov chaw huv si rau PCR teeb tsa thiab lwm yam kev cai rhiab.

 

Tus neeg siv khoom phooj ywg
Peb cov chaw ua haujlwm PCR tau nruab nrog UV cua recirculation, uas tswj cov laminar airflow kom tsis txhob muaj cov kab mob reagent. Nyob rau tib lub sijhawm, lawv tso saib lub sijhawm khiav ntawm lub raj ntim thiab lim ntawm lub vijtsam rau lub sijhawm hloov kho kom muaj lub zog tas li lub raj ntim.

 

Txuag tau yooj yim
Cov cuab yeej siv cov khoom siv hluav taws xob muab qhov chaw cia ntxiv rau cov tshuaj tiv thaiv kab mob, cov kav dej lossis cov khib nyiab saum lub nraub qaum. Cov kev faib tawm no tseem tuaj yeem tuav cov khoom siv hauv rooj zaum, tso cai rau ntau cov kauj ruam ua haujlwm sib koom ua ke yam tsis hloov txoj haujlwm los txo qhov kev pheej hmoo ntawm kev sib kis.

 

Zoo Compatibility
Peb cov chaw ua haujlwm PCR kuj tau nruab nrog ntau lub tshuab hluav taws xob sab hauv thiab sib haum nrog cov khoom siv hauv chav kuaj xws li cov centrifuges mini lossis vortex mixers, tso cai rau kev sib xyaw ua ke ntawm ntau cov kauj ruam ua haujlwm yam tsis cuam tshuam kev ua haujlwm.

 

Daim ntawv thov ntawm PCR System Construction Workstation
 
Pre Electrophoresis Loading Workstation

PCR chaw ua haujlwm yog tsim los tswj cov huab cua ncig kom txo tau cov kev sib kis kab mob uas tuaj yeem ua rau tsis raug. Cov yam ntxwv uas suav nrog hauv PCR chaw ua haujlwm yog:
● Ib cheeb tsam ua haujlwm tsis muaj menyuam rau cov txheej txheem aseptic
● Lub kiv cua ua haujlwm siab siv los ntawm lub tshuab hluav taws xob sib hloov (EC) lub cev muaj zog
● Kev txuag hluav taws xob LED teeb pom kev zoo Vertical laminar airflow nrog multiplex HEPA pom tshuab
● UV teeb rau kom tsis muaj menyuam

Muaj ntau yam kev siv ntawm PCR chaw ua haujlwm raws li teev hauv qab no:
● Kev hloov kho ntawm DNA tawg
● Lub amplification ntawm noob fragments raws li ib tug lwm txoj rau cloning
● Kev ntsuam xyuas DNA ntawm archaeological specimens
● Kev txheeb xyuas qhov tseeb ntawm cov kab mob pathogenic
● Kev tshawb pom ntawm kev hloov pauv uas cuam tshuam rau cov kab mob qub txeeg qub teg, cov ntaub so ntswg typing, lossis malignant transformation
● Kev ntsuam xyuas cov cim caj ces rau kev ua txhaum cai thiab daim ntawv thov forensic
● Kev kawm txog cov noob caj noob ces

Serial Dilution Workstation

 

 
Common PCR yog dab tsi?
 
01/

Multiplex PCR
Multiplex PCR ntiav cov khub primer sib txawv nyob rau hauv tib cov tshuaj tiv thaiv rau ib txhij amplification ntawm ntau lub hom phiaj. Piv nrog cov txheej txheem PCR siv tsuas yog 2 primers, ib qho kev sib tw ntxiv ntawm multiplex PCR yog qhov sib txawv hybridization kinetics ntawm txawv primer khub. Primers uas khi nrog kev ua haujlwm siab tuaj yeem siv ntau dua ntawm cov tshuaj tiv thaiv PCR, yog li txo cov txiaj ntsig ntawm lwm cov khoom PCR. Qhov no feem ntau ua rau cov DNA tsis sib luag thiab tsis muaj cov khoom PCR xav tau.

02/

Ntev-ntau PCR
PCR cov khoom uas muaj txog li 4 kb tuaj yeem ua raws li niaj hnub siv cov txheej txheem PCR raws tu qauv siv Taq DNA polymerase. Txawm li cas los xij, kev nthuav dav ntawm PCR cov khoom siv ntev dua 4 kb feem ntau ua tsis tau yam tsis muaj qhov ua kom zoo dua. Tiv thaiv DNA kev puas tsuaj, xws li DNA depurination, yog qhov tseem ceeb tshwj xeeb rau kev nthuav dav ntawm cov khoom PCR ntev, vim tias ib qho DNA lesion nyob rau hauv lub template yog txaus los tso tseg PCR enzyme. DNA puas thaum lub sij hawm PCR cycling tuaj yeem txo qis nrog cov tshuaj buffering tshwj xeeb uas tswj cov pH ntawm cov tshuaj tiv thaiv.

03/

Ib leeg-cell PCR
Ib leeg-cell PCR muab cov cuab yeej muaj txiaj ntsig rau kev txheeb xyuas caj ces siv cov khoom pib tsawg. Los ntawm ntws cytometry los yog micromanipulation, tus neeg lub hlwb ntawm kev txaus siab tuaj yeem raug cais raws li cov cim ntawm tes los yog lub cev. Kev nthuav dav ntawm cov qauv qis qis qis - uas tuaj yeem qis li ib lossis ob daim ntawv theej - yuav tsum muaj PCR system uas muaj txiaj ntsig zoo, tshwj xeeb, thiab rhiab heev.

04/

Methylation-specific PCR (MSP)
MSP tso cai rau cov txheej txheem methylation ntawm lub hom phiaj DNA los txiav txim siab tom qab kho sodium bisulfite. Txoj kev xav tau ob pawg ntawm primers los tsim: ib txheej uas anneals rau unchanged cytosines (piv txwv li, methylated nyob rau hauv lub genomic DNA) thiab ib txheej uas anneals rau uracil tshwm sim los ntawm bisulfite kev kho mob ntawm cytosines tsis methlyated nyob rau hauv lub genomic DNA. Amplification cov khoom tau muab los ntawm cov primer teem rau qhov tsis hloov pauv qhia tau hais tias cytosines tau methylated thiab yog li tiv thaiv los ntawm kev hloov pauv.

 

Hom PCR System Workstation
 

Muaj ob hom PCR chaw ua haujlwm raws li teev hauv qab no:

Laminar Flow

Laminar ntws, uas tas li lim thiab circulates cua thiab txav nws smoothly nyob rau hauv parallel txheej. PCR chaw ua haujlwm hom no ncig thiab lim cua tsis tu ncua hauv cov khaubncaws sab nraud povtseg. Siv cov txheej txheem HEPA filtration, PCR Laminar Flow Cabinet muab cov huab cua huv si nrog lub sijhawm UV teeb kom tsis muaj menyuam kom tiv thaiv ntau tshaj ntawm cov qauv paug. Yog tias koj tsa ob-fold sash, nws yuav tua lub teeb UV thiab ua rau lub tshuab ua haujlwm.

Dead Air

Qhov tuag Air PCR chaw ua haujlwm txhawb nqa huab cua tsis muaj cua. Dead Air PCR workstation unit tuaj nrog UV-lub teeb timer uas tso cai rau kom tsis muaj menyuam ntawm kev nthuav dav. Lub xub ntiag ntawm kev ruaj ntseg interlock hloov tiv thaiv kom raug UV teeb thaum koj tsa lub sash. Laminar flow txees ntiav HEPA huab cua pom thiab UV lub teeb kom tsis muaj menyuam kom tiv thaiv kev sib kis ntau tshaj plaws. Cov huab cua tshaj tawm hauv lub txee laminar ntws yuav tsum nyob nruab nrab ntawm 0.3 m / s thiab 0.5 m / s, nrog 240 mus rau 360 cua hloov txhua teev.

 

Qhov txawv ntawm PCR vs. Rapid COVID-19 Test

 

 

Muaj ob hom kev ntsuas COVID-19: qhov kev sim tshuaj polymerase saw tshuaj tiv thaiv (PCR) thiab cov tshuaj tiv thaiv kab mob, hu ua qhov kev sim sai.
PCR Test
● Nws ua haujlwm li cas
Kev kuaj PCR siv cov qauv ntawm ribonucleic acid (RNA) thiab "amplifies" nws nrog kev pab los ntawm lab technologies. Amplifying RNA pab ua kom cov kab me me ntawm COVID-19 tus kab mob pom hauv cov qauv kuaj. Txawm hais tias koj muaj kab mob me me hauv koj lub cev, PCR kuaj yuav kuaj pom nws.
● Cov txiaj ntsig
Kev kuaj PCR muaj kev ntseeg siab dua thiab muaj tseeb vim yog kuaj cov khoom siv caj ces ntawm tus kab mob, tshem tawm kev cuam tshuam los ntawm lwm yam kab mob.
● Cov tsis zoo
Thaum kuaj PCR yog txoj hauv kev zoo tshaj plaws los kuaj xyuas COVID-19, nws yuav tsum ua los ntawm tus kws kho mob. Cov kev ntsuam xyuas no tsis tsim cov txiaj ntsig tam sim ntawd, uas txhais tau tias koj yuav tau tos ob peb hnub kom paub seb koj puas zoo lossis tsis zoo.

Antigen Test
● Nws ua haujlwm li cas
Kev kuaj sai, hu ua antigen test, kuaj xyuas cov proteins uas pom nyob sab nraud ntawm tus kab mob. Kev kuaj antigen ua raws li cov tshuaj tiv thaiv kab mob ua. Cov tshuaj tiv thaiv yog "cov neeg zoo" uas txuas rau cov tshuaj tiv thaiv antigen (cov "cov neeg phem") thiab tua tus kabmob. Thaum koj muab koj tus nasal swab qauv, nws muab tso rau hauv ib lub kua thiab tom qab ntawd mus rau ib qho kev xeem strip.
● Cov txiaj ntsig
Cov txiaj ntsig hauv li 15 feeb.
Yooj yim txaus ua koj tus kheej hauv tsev. Ib txwm muaj chav me me rau cov neeg siv yuam kev, tab sis tsuav yog koj ua raws li cov lus qhia, txhua tus tuaj yeem kuaj lawv tus kheej nrog kev kuaj antigen.
Pab qeeb kis tus kab mob sib kis zoo li COVID-19 nrog cov txiaj ntsig sai dua
● Cov tsis zoo
Tsis zoo li PCR kuaj, kev kuaj antigen tsuas tuaj yeem txiav txim siab seb koj puas muaj tus kab mob hauv koj lub cev. Kev kuaj sai sai tsis tuaj yeem kuaj pom tus kab mob me me lossis cov tsos mob asymptomatic raws li qhov kev kuaj PCR tuaj yeem ua tau.

 

Tej yam uas yuav tsum tau xav txog thaum xaiv PCR System Kev Tsim Kho Workstation
1 Channel Automatic Workstaion
Liquid Handling Automated Workstation
Pipetting Robots
Automated Liquid Handler

PCR chaw ua haujlwm yog cov khoom siv ruaj khov uas yuav nyob hauv koj lub chaw kuaj mob ntev. Yog vim li cas thiaj xaiv qhov zoo tshaj plaws thaum xaiv qhov chaw ua haujlwm yuav khoom yog qhov tseem ceeb. Cov hauv qab no yog yam uas koj yuav tsum xav txog:

Hom haujlwm twg koj xav tau?
PCR workstations tuaj nyob rau hauv ob hom:
● UV chaw ua haujlwm
● UV + HEPA chaw ua haujlwm
Ob hom chaw ua haujlwm siv UV cov tshuaj tua kab mob los pab xyuas kom meej tias koj lub chaw ua haujlwm tsis muaj kab mob uas tuaj yeem los ntawm cov qauv yav dhau los lossis tib neeg. Qhov txawv yog tias qee qhov chaw ua haujlwm tuaj nruab nrog cov cua HEPA lim cua lim cua ua ntej nws nkag mus rau hauv chav ua haujlwm. Qhov ntawd tso cai rau cov neeg siv tsim ib puag ncig zoo uas pab ua kom cov qauv tsis muaj paug.

Yuav Ua Li Cas Zoo Siab Ua Rau PCR Workstation?
Lub siab zoo txhais tau hais tias huab cua siab hauv koj lub chaw ua haujlwm yog siab dua sab nraum nws chav. Qhov ntawd txhais tau hais tias huab cua tsuas tuaj yeem ntws tawm ntawm lub chamber, thiab huab cua los ntawm ib puag ncig sab nraud-uas tuaj yeem muaj cov khoom siv los yog cov kab mob tsis tuaj yeem ntws mus rau hauv qhov chaw ua haujlwm. Nyob rau tib lub sijhawm, lim cua hauv lub chaw ua haujlwm chamber raug tshuab tawm, nqa cov khoom paug.
Yuav kom tswj tau qhov zoo siab, cov chaw ua haujlwm yuav tsum muaj HEPA pom, airtight seals, thiab kiv cua uas pab cov neeg ua haujlwm tswj huab cua hauv chav tsev thaum twg lub chaw ua haujlwm siv.

Puas yog Dead Air cuam tshuam PCR cov txiaj ntsig?
PCR chaw ua haujlwm uas tsis muaj HEPA lim lossis kiv cua siv tuag lossis tseem huab cua. Qhov ntawd txhais tau hais tias huab cua hauv qhov chaw ua haujlwm tseem nyob ruaj khov hauv lub chamber. Tsis muaj cua txav hauv qhov chaw ua haujlwm, thiab tsis muaj cua tshuab rau hauv lossis tawm ntawm qhov chaw ua haujlwm. Qhov ntawd txo qis kev cuam tshuam rau cov qauv hauv lub chamber, tiv thaiv kev sib kis thiab ua kom cov khoom tsis muaj menyuam.
Vim tias tsis muaj qhov cua tshuab, cov cua tshuab tseem tuaj yeem tswj tsis tau qhov kub thiab txias thoob plaws hauv chav ua haujlwm. Ntau qhov chaw ntawm lub chamber tuaj yeem muaj qhov hloov pauv me ntsis. Qhov kub thiab txias tuaj yeem sib txawv raws li txheej hauv lub chamber. Txawm hais tias qhov sib txawv yuav me me, lawv tuaj yeem cuam tshuam qhov txiaj ntsig PCR.

PCR txo tus nqi ntawm lub sijhawm thiab nyiaj txiag
PCR chaw ua haujlwm yog ib qho chaw zoo rau kev tswj hwm DNA thiab RNA thiab teeb tsa PCR kev soj ntsuam. Lawv pab tiv thaiv kev kis kab mob uas tuaj yeem ua rau cov txiaj ntsig tsis tseeb lossis tsis tseeb, uas tuaj yeem siv sijhawm thiab nyiaj txiag.
Hauv luv luv, cov chaw ua haujlwm txo qis kev pheej hmoo ntawm kev sib kis los ntawm kev muab "chav sib cais" rau PCR cov tshuaj tiv thaiv. Lawv muab kev tswj hwm ib puag ncig rau PCR thiab RNA daim ntawv thov uas tiv thaiv koj cov qauv thiab pab koj ua tiav cov txiaj ntsig zoo.

 

Cov lus qhia txog kev nyab xeeb thaum siv PCR chaw ua haujlwm

 

● Tsis txhob siv PCR chaw ua haujlwm los ua cov qauv biohazard. PCR chaw ua haujlwm tsuas yog tiv thaiv cov qauv hauv thaj chaw ua haujlwm los ntawm cov pa tawm sab nraud. Lawv tsis tiv thaiv tus neeg teb xov tooj.
● Tso cai rau lub voj voog tshem tawm. Cia lub tshuab ziab khaub ncaws tsawg kawg 3 feeb ua ntej thiab tom qab siv los ntxuav qhov chaw ua haujlwm ntawm cov kab mob.
● Tsuas yog caj npab thiab txhais tes tso cai rau hauv thaj chaw ua haujlwm. Cov neeg ua haujlwm yuav tsum nco ntsoov nco ntsoov tias lub taub hau thiab lub xub pwg nyom yuav tsum tsis txhob nyob hauv thaj chaw ua haujlwm thiab ua li ntawd tej zaum yuav ua rau muaj kab mob.
● Tsis txhob tsuag tshuaj rau ntawm phab ntsa tom qab. Qhov no tuaj yeem ua rau lub lim dej HEPA / ULPA puas thiab ua rau cov dej xau.
● Tsis txhob tso koj lub cev ncaj qha rau saum cov qauv rhiab. Qhov no tej zaum yuav yog ib qho ua rau cov khoom sib kis.
● Tsis txhob khaws koj cov khoom hauv qhov chaw ua haujlwm. Kev thauj khoom ntau dhau ntawm qhov chaw ua haujlwm nrog cov khoom tsis tsim nyog tuaj yeem cuam tshuam rau qhov chaw ua haujlwm qhov chaw ua haujlwm huab cua thiab ntim.
● Tsuas yog cov ntaub ntawv tsis muaj menyuam yuav tsum muab tso rau hauv qhov chaw ua haujlwm huv. Xyuas kom tseeb tias tag nrho koj cov khoom raug tshuaj tua kab mob kom tsis txhob muaj kev sib kis ntawm lub rooj zaum ua haujlwm.
● Txheeb xyuas qhov chaw tsim nyog rau qhov chaw ua haujlwm. Kev cuam tshuam ntawm huab cua sab nraud (lub qhov rooj, kev tsheb ntau dhau ntawm tib neeg, qhov rais, diffusers, cua txias qhov hluav taws xob) tuaj yeem cuam tshuam qhov ntim. Txo qhov cuam tshuam rau airflow barrier.
● Tsis txhob siv lub tshuab hluav taws xob Bunsen hauv qhov chaw ua haujlwm. Qhov tshwm sim buoyancy tshwm sim yuav cuam tshuam rau qhov chaw ua haujlwm qhov chaw ua haujlwm airflow thiab ntim. Thaum tsim nyog, tsawg tus tsav lub teeb hom hluav taws xob burners yuav siv tau.
● Hnav Cov Khoom Tiv Thaiv Tus Kheej (PPE) kom raug. Hnav ib lub tsho tiv thaiv rov qab (los tiv thaiv tus neeg teb xov tooj los ntawm kev sib tsoo) nrog rau ob lub hnab looj tes (dhau lub cuffs) yuav tsum tau xyaum.

 

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Cov lus nug nquag nug ntawm PCR System Kev Tsim Kho Chaw Ua Haujlwm

 

Q: PCR workstation yog dab tsi?

A: PCR chaw ua haujlwm yog tsim los tiv thaiv kev kis kab mob thaum lub sij hawm rhiab PCR amplification thiab manipulation ntawm DNA los yog RNA. Txij li thaum txo qis kev sib kis kab mob ua rau txo qis cov txiaj ntsig tsis raug, PCR cov txee thiab cov hoods yog qhov tseem ceeb ntxiv rau cov khoom siv molecular biology thiab genomics lab.

Q: PCR sawv cev rau dab tsi?

A: Polymerase saw cov tshuaj tiv thaiv. Polymerase saw cov tshuaj tiv thaiv (abbreviated PCR) yog cov txheej txheem sim ua kom sai sai (amplifying) lab mus rau billions ntawm cov ntawv luam ntawm ib ntu ntawm DNA, uas tuaj yeem kawm paub ntau ntxiv.

Q: Qhov txawv ntawm PCR workstation thiab biosafety txee yog dab tsi?

A: Lub PCR Laboratory hood zoo li lub laminar huv lub rooj ntev zaum, qhov twg cov cua lim ntws los thiab mus rau tus neeg siv. Qee lub sij hawm cov chav kuaj hoods no yog kev ncig tsis pub dawb xws li lub thawv cua tuag. Txawm li cas los xij, qhov sib txawv ntawm qhov no yog kev siv UV sterilization koj yuav pom hauv lub txee biosafety.

Q: Lub hom phiaj ntawm lub txee PCR yog dab tsi?

A: Polymerase Chain Reaction (PCR) txee muab DNA thiab RNA tsis muaj paug ib puag ncig los ntawm HEPA lim thiab UV decontamination system. Nws yog ib feem tseem ceeb ntawm PCR lub chaw soj nstuam uas xav tau cov ntawv thov xws li kev npaj PCR mastermix thiab UV decontamination ntawm PCR consumables.

Q: Puas yog PCR chaw ua haujlwm tsis muaj menyuam?

A: PCR chaw ua haujlwm tau tsim los txo qhov kev pheej hmoo ntawm kev sib kis thaum ua cov kev sim Polymerase Chain Reaction (PCR). Cov chaw ua haujlwm no muab qhov chaw tswj hwm thiab tsis muaj menyuam los ntawm kev txo cov pa paug hauv huab cua thiab ua kom tsis muaj menyuam ua haujlwm thiab cov cuab yeej nruab nrab ntawm kev nthuav dav.

Q: Koj hu li cas lub tshuab PCR?

A: Lub thermal cycler (tseem hu ua thermocycler, PCR tshuab lossis DNA amplifier) ​​yog lub tshuab kuaj xyuas feem ntau siv los nthuav cov ntu ntawm DNA ntawm cov tshuaj tiv thaiv polymerase saw (PCR). Nws ua kom sov thiab txias cov qauv hauv cov voj voog rov qab los pab txhawb DNA lossis RNA kev nthuav dav los ntawm cov tshuaj tiv thaiv polymerase saw.

Q: Lub tshuab PCR puas tuaj yeem kis tau?

A: Amplicons tuaj yeem ua tus qauv rau PCR cov tshuaj tiv thaiv yav tom ntej thiab yog li tuaj yeem kis tau yooj yim PCR lossis cov qauv npaj cov tshuaj, khoom siv, lossis khoom siv. Qhov no txhais tau hais tias cov khoom siv thiab PPE (lab coats, hnab looj tes, tsom iav, thiab lwm yam).

Q: Lub hauv paus ntsiab lus ntawm PCR txee yog dab tsi?

A: PCR txee yog nyob rau hauv txoj cai tib yam li lub ntsug laminar flow txee, tab sis nrog ob peb kev hloov kho: Txo qhov siab: thaum ua PCR, koj tsis xav tau ntau qhov siab. Qhov tseeb, kev ua haujlwm nyob ib puag ncig PCR koom nrog feem ntau yog pipetting, uas yog ua tiav ntawm qhov siab ntawm qhov chaw ua haujlwm. Optimized rau ceev UV decontamination.

Q: Lub hom phiaj ntawm PCR yog dab tsi thiab peb cov kauj ruam cuam tshuam li cas?

A: PCR yog ua raws li peb cov kauj ruam yooj yim uas yuav tsum tau ua rau txhua qhov DNA synthesis cov tshuaj tiv thaiv: denaturation ntawm tus qauv rau hauv ib leeg; annealing ntawm primers rau txhua tus thawj strand rau tshiab strand synthesis; thiab txuas ntxiv ntawm DNA tshiab strands los ntawm primers.

Q: Dab tsi yog peb chav kawm ntawm biosafety txee?

A: Muaj peb chav BSCs: Chav I, II thiab III. Thaum tag nrho peb chav kawm them taus cov neeg ua haujlwm thiab kev tiv thaiv ib puag ncig, tsuas yog Chav Kawm II thiab III cov khoom siv tiv thaiv. Class I BSCs yog tsim nyog rau kev ua hauj lwm nrog rau cov neeg ua hauj lwm uas muaj kev pheej hmoo tsawg rau nruab nrab.

Q: Dab tsi yog qhov feem ntau siv biosafety txee?

A: Cov txee feem ntau yog Chav Kawm II Hom A2 biosafety txee, txawm tias muaj ntau yam khoom siv cua tshuab. Chav Kawm II Hom A2 biosafety txee yog cov txee ntau tshaj plaws hauv tsev kawm ntawv. Nws siv cov ntaub thaiv huab cua thiab HEPA lim kom muab ob qho tib si ntim thiab ib puag ncig tsis muaj menyuam.

Q: Lwm lub npe rau lub txee biosafety yog dab tsi?

A: Thaum koj taug kev mus rau hauv chav kuaj kev tshawb fawb, muaj ib qho khoom siv uas feem ntau raug xa mus los ntawm ntau lub npe: cell culture hood, tissue culture hood, laminar flow hood, PCR hood, clean bench, or biosafety txee.

Q: Qhov zoo ntawm PCR yog dab tsi?

A: PCR kuj tau siv thoob plaws hauv thaj chaw ntawm molecular biology, pab cov kws tshawb fawb clone thiab cov noob caj noob ces txhawm rau txhawm rau tshawb pom kev hloov pauv. Tsis ntev los no nws tau dhau los ua qauv rau kev kuaj xyuas cov kab mob microbial, tsuas yog xav tau ib qho qauv me me rau kev tshuaj xyuas.

Q: 4 kauj ruam ntawm PCR yog dab tsi?

A: Cov txheej txheem PCR muaj 4 kauj ruam: sau, npaj, nthuav dav, thiab tom qab PCR ntxuav. PCR tshuab cov kauj ruam tshwm sim nyob rau hauv cov kauj ruam amplification. Nws pib nrog ib ntu ntawm cov qauv DNA muab tso rau hauv lub raj tsim nyog nrog rau cov tshuaj reagents thiab tshuaj uas tau teev tseg saum toj no.

Q: Yam khoom twg kuv xav tau rau PCR?

A: Muaj ntau yam khoom tsim los pab txhawb cov txheej txheem polymerase chain reaction (PCR). Cov khoom muaj xws li thermal cyclers, cov khoom siv, PCR reagents, oligonucleotides, dNTPs, master mixes, enzymes, custom primers, arrays, assay, dyes, standard, accessories, etc.

Q: Dab tsi yog qhov tsis zoo ntawm PCR?

A: Qhov tsis zoo ntawm PCR thev naus laus zis:
Kev kis kab mob:Cov txheej txheem PCR muaj qhov cuam tshuam rau kev sib kis los ntawm lwm qhov chaw ntawm DNA lossis RNA lossis ib puag ncig.
Nqi thiab complexity:PCR tuaj yeem kim thiab xav tau cov kws tshaj lij kev paub rau kev ua haujlwm siab.
Ntau yam khoom.

Q: Dab tsi yog qhov teeb meem ntawm PCR tshuab?

A: Yog tias cov raj raug tshem tawm ua ntej lub tshuab txias lub sij hawm cov lus qhia ntiv tes tuaj yeem raug mob me me. Tiv tauj nrog cov cua sov thaiv thaum kub kub thaum lub sij hawm thermal (xws li ntxiv polymerase rau 'hotstart' PCR). Tsis quav ntsej cov lus ceeb toom thaum tshav kub kub Tiv tauj nrog lossis raug tshav kub lossis txias me me: kub hnyiab rau tes / ntiv tes.

Q: Dab tsi yuav tsum tau ceev faj thaum lub tshuab PCR?

A: Siv hnab looj tes txhua lub sijhawm thiab tsis txhob teeb tsa PCR cov tshuaj tiv thaiv ze ntawm thaj chaw uas yog siv los khiav PCR cov khoom ntawm cov gel. Tsis tas li ntawd, tsis txhob siv tib lub pipettes rau thauj cov gel thiab teeb tsa PCR. PCR cov khoom aerosols tuaj yeem nkag mus rau hauv cov kav dej pipette thiab yog li kis tau rau PCRs tom ntej.

Q: Dab tsi yog cov tshuaj tua kab mob siv hauv cov khoom siv biosafety?

A: Decontamination thiab ntxuav. Decontaminate BSC ua ntej thiab tom qab txhua qhov kev siv los tiv thaiv kev sib kis. Ib txoj hauv kev yog siv 1:10 cov tshuaj bleach tshiab ua raws li 70% ethanol yaug kom tsis txhob xeb thiab ua kom muaj tshuaj tua kab mob zoo. Tiv tauj peb yog tias koj xav tau ib qho tshuaj dawb.

Q: Lub hom phiaj ntawm PCR tsis yog dab tsi?

A: Cov tsis tswj pH ntawm cov tshuaj tiv thaiv sib tov thiab muab cov poov tshuaj (K +) lossis ammonium (NH4+) ions rau kev ua haujlwm zoo enzyme . Cov ions no txhawb nqa qhov kev tshem tawm ntawm cov primers los ua qauv DNA, ua kom tsis muaj kev ruaj ntseg ntawm kev sib khi ntawm cov primers thiab cov cheeb tsam tawm tsam, thiab nce PCR tshwj xeeb.

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