Lub chaw kuaj ntshav molecular ntawm Tuam Tshoj Agricultion University (CE) Qaug zog thiab ua haujlwm tsis raug .
Prcxi Bieworkstation Tag Nrho Cov Txheej Txheem Empowerment:
Txhawm rau txhawm rau hla cov hliav tshuaj thiab txhim kho kev ua haujlwm thiab cov ntaub ntawv pov thawj ntawm kev sim ua ntej lossis cov ntaub ntawv tsis siv neeg nyob hauv ib qho kev tshawb fawb prcxi (CE) 20 teev ntawm prcxi pipetting workstations .
Txheej txheem cej luam ntawm cov txheej txheem rau hom kev sim no:
(Nco tseg: Vim yog kev zais ntshis ntawm cov ntsiab lus ntawm cov neeg siv khoom, tsis yog tus txheej txheem tiag tiag raws li cov neeg siv khoom, thiab tsuas yog rau kev siv ntawm cov neeg siv khoom ntawm tib hom)
Khoom npaj khoom:
Prcxi Pipetting Workstation, 96- e}} Daim phaj zoo, cov lus qhia, thav duab sib nqus, PCROINS
Cov txheej txheem sim:
Ntxiv 100 μl ntawm cov qauv thiab 100 μL ntawm Lysis Buffner rau txhua qhov zoo ntawm {2} siv lub workstation .
② Vortex cov kev sib nqus
③ Strcubate rau tsawg kawg 2 feeb kom tso cai ua tiav kev khi ntawm cov hlaws nplaum ua kom zoo nkauj rau nucleic acids {}}
④ Muab cov 96-}} zoo phaj ntawm cov xim sib nqus, cia cov neeg sib nqus, cia li muab cov khoom sib nqus rau thaum lub caij thaum sawv ntxov.
Tshem tawm cov phaj los ntawm cov xim sib nqus, ntxiv ntxuav ua kom zoo, sib tov los ntawm pipetting thiab sau 5 zaug, thiab ncu rau 2 feeb.
⑥ Muab cov phaj rov qab rau ntawm cov xim sib nqus, cia sawv ntsug li 1 feeb, thiab pov tseg cov supernatant .
Op tshem lub phaj los ntawm kev sib nqus muag zaub, ua kom huv tsis rau txhua qhov zoo, thiab sib tov los ntawm pipetting nce thiab nqis 5 zaug.
⑧ Muab cov phaj ntawm cov xim sib nqus, cia sawv ntsug li 1 feeb, thiab pov tseg cov supernatant .
⑨ Ntxiv ntxuav ua dab tsi rau txhua qhov tshuaj tiv thaiv zoo, sib xyaw los ntawm pipetting nce thiab nqis 3 zaug, thiab ncu rau 1 feeb.
⑩ Hloov cov phaj mus rau lub sib nqus sawv, cia nws khom rau 1 feeb, tom qab ntawd supernatant rau lub nucleic acids) .
Cov khoom lag luam no siv cov hniav sib tsoo lossis cov khoom siv roj ntsha uas tsis muaj txiaj ntsig zoo li cov nroj tsuag ntawm cov lysins thiab cov protein thiab cov roj ntsha. Thaum kawg, Cov hlaws dai nucleic tau els los ntawm cov ntawv tso cai sib nqus uas siv cov kev siv sib nqus ntawm cov ntawv sau ua ke, suav nrog cov ntawv sau ua kom huv si rau cov ntawv sau cia rau hauv, suav nrog cov ntawv sau ua kom huv si rau cov ntawv sau ua ke, suav nrog kev siv cov kua qaub rau cov dej khov, nrog rau kev siv nyiaj rau puring, xws li .
Cov qhua tuaj noj mov
Lub pipetting workstation los ntawm Prcxi tau txhim kho qhov ua tau zoo, tau zoo} Lub siab ua kom ruaj khov, thiab kev ntseeg tau zoo ntawm kev sim ua.
Tam sim no, lub nucleic acid extraction ua haujlwm hauv chav kuaj tau ua kom muaj txiaj ntsig ntxiv ntawm qoob loo ntxiv rau kev txhim kho kev txhim kho kev ua kom zoo dua .